The estimation of glutathione in rat tissues. A comparison of a new spectrophotometric method with the glyoxalase method.
نویسندگان
چکیده
The method for the estimation of oxidized and reduced glutathione (GSSG and GSH) based on the glyoxalase reaction (Woodward, 1935; Martin & Mcllwain, 1959) is the only method available with a high degree of specificity. The present paper suggests another specific method based on thioldisulphide exchange reactions. Pihl, Eldjarn & Bremer (1957) have shown that exchange reactions between GSH and certain disulphides result in the formation of GSSG, which then reacts with NADPH2 in the presence of glutathione reductase:
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عنوان ژورنال:
- The Biochemical journal
دوره 93 2 شماره
صفحات -
تاریخ انتشار 1964